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CD40 Ligand antibody (AA 47-261)

Name: CD40 Ligand antibody (AA 47-261)
Brand: antibodies-online
SKU: ABIN5611141
Price: 533.5 USD
Availability: InStock

CD40LG Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 8H10F5 unconjugated

Western blot analysis using CD154 mAb against HEK293 (1) and CD154 (AA: extra 47-261)-hIgGFc transfected HEK293 (2) cell lysate. (CD40 Ligand antibody (AA 47-261))

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng) (CD40 Ligand antibody (AA 47-261))

ELISA

Western blot analysis using CD154 mAb against human CD154 (AA: extra 47-261) recombinant protein. (CD40 Ligand antibody (AA 47-261))

Flow cytometric analysis of Ramos cells using CD154 mouse mAb (green) and negative control (red). (CD40 Ligand antibody (AA 47-261))

FACS

4 images

Catalog No. ABIN5611141

Datasheet as PDF

Target See all CD40 Ligand (CD40LG) Antibodies

CD40 Ligand (CD40LG)
Binding Specificity
16

10

8

7

6

4

4

4

4

4

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 47-261
Reactivity
144

80

34

9

8

6

5

2

2

2

2

1

1

1

1
Human
Host
90

65

21

15

11

1
Mouse
Compatible Secondaries
Clonality
111

86

4
Monoclonal
Conjugate
102

23

21

14

8

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD40 Ligand antibody is un-conjugated
Application
108

99

53

49

39

29

20

16

15

14

13

13

4

4

4

4

3

3

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), ELISA

Purpose

CD154 Antibody

Purification

Purified antibody

Immunogen

Purified recombinant fragment of human CD154 (AA: 47-261) expressed in E. Coli.

Clone

8H10F5

Isotype

IgG1

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anti-CD40 Ligand (CD40LG) (AA 1-261) antibody
CD40LG Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) (AA 108-261) antibody
CD40LG Reactivity: Human FACS, IHC, ELISA, IF, Coat, StM Host: Mouse Monoclonal CD40LG-2761 unconjugated

anti-CD40 Ligand (CD40LG) (AA 108-261) antibody
CD40LG Reactivity: Human FACS, IHC, ELISA, IF, Coat, StM Host: Mouse Monoclonal CD40LG-2763 unconjugated

anti-CD40 Ligand (CD40LG) antibody (FITC)
CD40LG Reactivity: Mouse FACS Host: Armenian Hamster Monoclonal MR-1 FITC

anti-CD40 Ligand (CD40LG) (AA 33-62), (N-Term) antibody
CD40LG Reactivity: Human WB, FACS, IF, IHC (p) Host: Rabbit Polyclonal RB19150 unconjugated

anti-CD40 Ligand (CD40LG) (AA 79-129) antibody
CD40LG Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) (AA 33-62) antibody
CD40LG Reactivity: Human WB, FACS, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) (AA 111-261) antibody
CD40LG Reactivity: Pig WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) antibody (PE)
CD40LG Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal 24-31 PE

anti-CD40 Ligand (CD40LG) (AA 47-261) antibody
CD40LG Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 5A3A9 unconjugated

Application Notes

ELISA: 1/10000
FCM: 1/200 - 1/400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Target

CD40 Ligand (CD40LG)

Alternative Name

CD154 (CD40LG Products)

Synonyms

CD154 antibody, cd40l antibody, CD40L antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TNFSF5 antibody, TRAP antibody, gp39 antibody, hCD40L antibody, CD40-L antibody, Cd40l antibody, Ly-62 antibody, Ly62 antibody, Tnfsf5 antibody, CD40 ligand antibody, TNF superfamily member 5 antibody, CD40LG antibody, cd40l antibody, Cd40lg antibody

Background

Description: The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Aliases: CD40LG, IGM, IMD3, TRAP, gp39, CD40L, HIGM1, T-BAM, TNFSF5, hCD40L

Molecular Weight

29.3kDa

Gene ID

959

HGNC

959

UniProt

P29965

Pathways

NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints