Glutaminase antibody (AA 580-669)

Catalog No. ABIN561056

Product Details anti-Glutaminase Antibody

Target: Glutaminase (GLS)

Binding Specificity: AA 580-669

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glutaminase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: Mouse monoclonal antibody raised against a partial recombinant GLS.

Sequence: EQRDYDSRTA LHVAAAEGHV EVVKFLLEAC KVNPFPKDRW NNTPMDEALH FGHHDVFKIL QEYQVQYTPQ GDSDNGKENQ TVHKNLDGLL

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: GLS (NP_055720, 580 a.a. ~ 669 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 5C4

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-Glutaminase antibody (ABIN561056)

Reynolds, Lane, Robertson, Kemp, Liu, Hill, Dean, Clem: "Control of glutamine metabolism by the tumor suppressor Rb." in: Oncogene, Vol. 33, Issue 5, pp. 556-66, (2014) (PubMed).

Hida, Mouri, Ando, Mori, Mamiya, Iwamoto, Ozaki, Yamada, Nabeshima, Noda: "Combination of neonatal PolyI:C and adolescent phencyclidine treatments is required to induce behavioral abnormalities with overexpression of GLAST in adult mice." in: Behavioural brain research, Vol. 258, pp. 34-42, (2013) (PubMed).

Cassago, Ferreira, Ferreira, Fornezari, Gomes, Greene, Pereira, Garratt, Dias, Ambrosio: "Mitochondrial localization and structure-based phosphate activation mechanism of Glutaminase C with implications for cancer metabolism." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. 1092-7, (2012) (PubMed).

Unterluggauer, Mazurek, Lener, Hütter, Eigenbrodt, Zwerschke, Jansen-Dürr: "Premature senescence of human endothelial cells induced by inhibition of glutaminase." in: Biogerontology, Vol. 9, Issue 4, pp. 247-59, (2008) (PubMed).

Target Details for Glutaminase

Target: Glutaminase (GLS)

Alternative Name: GLS (GLS Products)

Background: Full Gene Name: glutaminase
Synonyms: AAD20,DKFZp686O15119,FLJ10358,GLS1,KIAA0838

Gene ID: 2744

NCBI Accession: NM_014905

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect