GAPDH antibody (AA 226-335)

Catalog No. ABIN560997

Product Details anti-GAPDH Antibody

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Binding Specificity: AA 226-335

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Mouse monoclonal antibody raised against a partial recombinant GAPDH.

Sequence: GKLTGMAFRV PTANVSVVDL TCRLEKPAKY DDIKKVVKQA SEGPLKGILG YTEHQVVSSD FNSDTHSSTF DAGAGIALND HFVKLISWYD NEFGYSNRVV DLMAHMASKE

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: GAPDH (NP_002037, 226 a.a. ~ 335 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 3C2

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-GAPDH antibody (ABIN560997)

Li, Xia, Ma, Zhang, Mao, Wang, Zhou, Lau, Ng: "miR-106b is overexpressed in medulloblastomas and interacts directly with PTEN." in: Neuropathology and applied neurobiology, Vol. 41, Issue 2, pp. 145-64, (2015) (PubMed).

Wu, Ke, Horng: "RacGTPase-activating protein 1 interacts with hepatitis C virus polymerase NS5B to regulate viral replication." in: Biochemical and biophysical research communications, Vol. 454, Issue 1, pp. 19-24, (2015) (PubMed).

Okita, Honda, Kishimoto, Liao, Azumi, Hashimoto, Matsumoto: "Supplementation of strontium to a chondrogenic medium promotes chondrogenic differentiation of human dedifferentiated fat cells." in: Tissue engineering. Part A, Vol. 21, Issue 9-10, pp. 1695-704, (2015) (PubMed).

Song, Ma, Cong, Shen, Jing, Ying, Zhou, Jiang, Fu, Yan: "Radioprotective effects of genistein on HL-7702 cells via the inhibition of apoptosis and DNA damage." in: Cancer letters, Vol. 366, Issue 1, pp. 100-11, (2015) (PubMed).

Wu, Ke, Hsu, Yeh, Horng: "Reticulon 3 interacts with NS4B of the hepatitis C virus and negatively regulates viral replication by disrupting NS4B self-interaction." in: Cellular microbiology, Vol. 16, Issue 11, pp. 1603-18, (2014) (PubMed).

Dai, Jiang, Li, Wang, Liu, Patankar, Zheng: "Expression and roles of Slit/Robo in human ovarian cancer." in: Histochemistry and cell biology, Vol. 135, Issue 5, pp. 475-85, (2011) (PubMed).

Otsuka, Takata, Yoshikawa, Kojima, Kishikawa, Shibata, Takekawa, Yoshida, Omata, Koike: "Receptor for activated protein kinase C: requirement for efficient microRNA function and reduced expression in hepatocellular carcinoma." in: PLoS ONE, Vol. 6, Issue 9, pp. e24359, (2011) (PubMed).

Arai, Hayashi, Sobou, Ishida, Kawachi, Kotoku, Kobayashi: "Anti-angiogenic effect of triterpenoidal saponins from Polygala senega." in: Journal of natural medicines, Vol. 65, Issue 1, pp. 149-56, (2010) (PubMed).

Takahashi, Grigliatti, Reid, Riggs: "The effect of allelic variation in aldo-keto reductase 1C2 on the in vitro metabolism of dihydrotestosterone." in: The Journal of pharmacology and experimental therapeutics, Vol. 329, Issue 3, pp. 1032-9, (2009) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH (GAPDH Products)

Synonyms: G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody

Background: Full Gene Name: glyceraldehyde-3-phosphate dehydrogenase
Synonyms: G3PD,GAPD,MGC88685

Gene ID: 2597

NCBI Accession: NM_002046