IFT74 antibody (C-Term) (Biotin)

Catalog No. ABIN5608778

Product Details anti-IFT74 Antibody (Biotin)

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IFT74 antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB)

Purpose: CMG1 / CCDC2 / IFT74, Biotinylated

Sequence: KTIVDALHST SGN.

Cross-Reactivity: Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2 kDa according to AAK77221.1). The observed molecular weight corresponds to earlier findings in literature (Momeni et al, BMC Neurol. 2006 Dec 13,6:44., PMID: 17

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IFT74

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Alternative Name: IFT74 (IFT74 Products)

Background: IFT74, CCDC2, CMG-1, FLJ22621, MGC111562, intraflagellar transport 74 homolog (Chlamydomonas), capillary morphogenesis protein 1, coiled-coil domain containing 2, CMG1

Gene ID: 80173, 313365

NCBI Accession: NP_001092692, NP_001092693, NP_079379

Pathways: Hedgehog Signaling, Chromatin Binding