Dihydrofolate Reductase antibody (AA 88-187)

Catalog No. ABIN560600

Product Details anti-Dihydrofolate Reductase Antibody

Target: Dihydrofolate Reductase (DHFR)

Binding Specificity: AA 88-187

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dihydrofolate Reductase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: Mouse monoclonal antibody raised against a partial recombinant DHFR.

Sequence: HFLSRSLDDA LKLTEQPELA NKVDMVWIVG GSSVYKEAMN HPGHLKLFVT RIMQDFESDT FFPEIDLEKY KLLPEYPGVL SDVQEEKGIK YKFEVYEKND

Cross-Reactivity: Human, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: DHFR (AAH03584, 88 a.a. ~ 187 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 2B10

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-Dihydrofolate Reductase antibody (ABIN560600)

Jabs, Oelze, Mikhed, Stamm, Kröller-Schön, Welschof, Jansen, Hausding, Kopp, Steven, Schulz, Stasch, Münzel, Daiber: "Effect of soluble guanylyl cyclase activator and stimulator therapy on nitroglycerin-induced nitrate tolerance in rats." in: Vascular pharmacology, Vol. 71, pp. 181-91, (2015) (PubMed).

Kossmann, Hu, Steven, Schönfelder, Fraccarollo, Mikhed, Brähler, Knorr, Brandt, Karbach, Becker, Oelze, Bauersachs, Widder, Münzel, Daiber, Wenzel: "Inflammatory monocytes determine endothelial nitric-oxide synthase uncoupling and nitro-oxidative stress induced by angiotensin II." in: The Journal of biological chemistry, Vol. 289, Issue 40, pp. 27540-50, (2014) (PubMed).

Oelze, Kröller-Schön, Welschof, Jansen, Hausding, Mikhed, Stamm, Mader, Zinßius, Agdauletova, Gottschlich, Steven, Schulz, Bottari, Mayoux, Münzel, Daiber et al.: "The sodium-glucose co-transporter 2 inhibitor empagliflozin improves diabetes-induced vascular dysfunction in the streptozotocin diabetes rat model by interfering with oxidative stress and ..." in: PLoS ONE, Vol. 9, Issue 11, pp. e112394, (2014) (PubMed).

Vodenicharov, Laterreur, Wellinger: "Telomere capping in non-dividing yeast cells requires Yku and Rap1." in: The EMBO journal, Vol. 29, Issue 17, pp. 3007-19, (2010) (PubMed).

Yoon, Choi, Kim, Shin, Zhang, Kang: "Influence of reduced folate carrier and dihydrofolate reductase genes on methotrexate-induced cytotoxicity." in: Cancer research and treatment : official journal of Korean Cancer Association, Vol. 42, Issue 3, pp. 163-71, (2010) (PubMed).

Ionova, Vásquez-Vivar, Whitsett, Herrnreiter, Medhora, Cooley, Pieper: "Deficient BH4 production via de novo and salvage pathways regulates NO responses to cytokines in adult cardiac myocytes." in: American journal of physiology. Heart and circulatory physiology, Vol. 295, Issue 5, pp. H2178-87, (2008) (PubMed).

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR (DHFR Products)

Synonyms: DHFR antibody, F10_orf160 antibody, ECK0049 antibody, JW0047 antibody, tmrA antibody, cb595 antibody, zgc:86637 antibody, DDBDRAFT_0187132 antibody, DDBDRAFT_0230113 antibody, DDB_0187132 antibody, DDB_0230113 antibody, CG14887 antibody, Dmel\\CG14887 antibody, dhfr antibody, DHFRP1 antibody, DYR antibody, 8430436I03Rik antibody, AA607882 antibody, AI662710 antibody, AW555094 antibody, Dhfr1 antibody, confers resistance to sulfonamides antibody, dihydrofolate reductase antibody, dihydrofolate reductase DhfR antibody, Dihydrofolate reductase antibody, dhfr antibody, DHFR antibody, dhfR antibody, folA antibody, dfrA antibody, LOC707382 antibody, CNJ01940 antibody, DDB_G0286755 antibody, Dhfr antibody, Mesil_0096 antibody, Arch_0408 antibody, Fbal_3367 antibody, Ilyop_0482 antibody, Lbys_2007 antibody, Riean_0733 antibody, Mrub_1855 antibody, Poras_0766 antibody, Halhy_3552 antibody, Mesop_3614 antibody, LOC609048 antibody

Background: Full Gene Name: dihydrofolate reductase
Synonyms:

Gene ID: 1719

Pathways: Mitotic G1-G1/S Phases