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p21 antibody (AA 65-164)

Name: p21 antibody (AA 65-164)
SKU: ABIN560309
Price: 574.67 USD
Availability: InStock

CDKN1A Reactivity: Human WB, ELISA, IF, PLA Host: Mouse Monoclonal 2F1 unconjugated

CDKN1A monoclonal antibody (M02), clone 2F1. (p21 antibody (AA 65-164))

Immunofluorescence of monoclonal antibody to CDKN1A on HeLa cell. (p21 antibody (AA 65-164))

Proximity Ligation Analysis of protein-protein interactions between CASP3 and CDKN1A. (p21 antibody (AA 65-164))

PLA

Western Blot detection against Immunogen (36. (p21 antibody (AA 65-164))

Detection limit for recombinant GST tagged CDKN1A is approximately 0. (p21 antibody (AA 65-164))

ELISA

5 images

(2 references)

Catalog No. ABIN560309

Datasheet as PDF

Target See all p21 (CDKN1A) Antibodies

p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
Binding Specificity
36

30

16

15

15

10

8

8

8

7

7

7

7

7

6

4

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 65-164
Reactivity
240

120

96

36

13

11

5

4

2

1

1

1

1

1

1
Human
Host
209

50

1
Mouse
Compatible Secondaries
Clonality
202

57

1
Monoclonal
Conjugate
104

17

15

15

10

8

5

5

5

5

5

5

5

5

5

5

5

3

3

3

3

3

3

3

3

3

3

3

3
This p21 antibody is un-conjugated
Application
158

92

67

66

66

55

52

41

41

33

19

13

13

5

2

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Proximity Ligation Assay (PLA)

Purpose

Mouse monoclonal antibody raised against a partial recombinant CDKN1A.

Sequence

WERVRGLGLP KLYLPTGPRR GRDELGGGRR PGTSPALLQG TAEEDHVDLS LSCTLVPRSG EQAEGSPGGP GDSQGRKRRQ TSMTDFYHSK RRLIFSKRKP

Cross-Reactivity

Human

Characteristics

Antibody Reactive Against Recombinant Protein.

Immunogen

CDKN1A (AAH00312.1, 65 a.a. ~ 164 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone

2F1

Isotype

IgG1

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CDKN1A Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

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CDKN1A Reactivity: Human, Non-Human Primate ELISA, IHC (p), ICC Host: Mouse Monoclonal WA-1 unconjugated

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CDKN1A Reactivity: Synthetic WB, IHC (f) Host: Rabbit Monoclonal unconjugated Recombinant Antibody

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 21-100) antibody
CDKN1A Reactivity: Human, Mouse, Rat, Sheep WB, ELISA, FACS, IHC (p), IF (cc), IF (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr145) antibody
CDKN1A Reactivity: Human, Mouse, Rat WB, ELISA, IF, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 134-164), (C-Term) antibody
CDKN1A Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB3180 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 117-146), (C-Term) antibody
CDKN1A Reactivity: Human, Mouse WB, IF, IHC (p) Host: Rabbit Polyclonal RB33282 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr145) antibody
CDKN1A Reactivity: Human WB, IHC (p), DB Host: Rabbit Polyclonal RB07512 unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Buffer

In 1x PBS, pH 7.4

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Massimi, Cardarelli, Galli, Giardi, Ragusa, Panera, Cinque, Cifone, Biagioni, Giorgi: "Increase of Intracellular Cyclic AMP by PDE4 Inhibitors Affects HepG2 Cell Cycle Progression and Survival." in: Journal of cellular biochemistry, Vol. 118, Issue 6, pp. 1401-1411, (2016) (PubMed).

Huang, Chuang, Hao, Talbot, Sen, Trink, Sidransky, Ratovitski: "Phospho-?Np63? is a key regulator of the cisplatin-induced microRNAome in cancer cells." in: Cell death and differentiation, Vol. 18, Issue 7, pp. 1220-30, (2011) (PubMed).
Target

p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name

CDKN1A (CDKN1A Products)

Background

Full Gene Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Synonyms: CAP20,CDKN1,CIP1,MDA-6,P21,SDI1,WAF1,p21CIP1

Gene ID

1026

NCBI Accession

NM_000389

Pathways

p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy