BUB1B antibody (AA 1-130)

Catalog No. ABIN560109

Product Details anti-BUB1B Antibody

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Binding Specificity: AA 1-130

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA), RNA Interference (RNAi)

Purpose: Mouse monoclonal antibody raised against a partial recombinant BUB1B.

Sequence: MAAVKKEGGA LSEAMSLEGD EWELSKENVQ PLRQGRIMST LQGALAQESA CNNTLQQQKR AFEYEIRFYT GNDPLDVWDR YISWTEQNYP QGGKESNMST LLERAVEALQ GEKRYYSDPR FLNLWLKLGR

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: BUB1B (AAH18739, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 2G5

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-BUB1B antibody (ABIN560109)

Gurvits, Repo, Löyttyniemi, Nykänen, Anttinen, Kuopio, Talvinen, Kronqvist: "Prognostic implications of securin expression and sub-cellular localization in human breast cancer." in: Cellular oncology (Dordrecht), Vol. 39, Issue 4, pp. 319-31, (2016) (PubMed).

Suzuki, Raya, Kawakami, Morita, Matsui, Nakashima, Gage, Rodríguez-Esteban, Izpisúa Belmonte: "Nanog binds to Smad1 and blocks bone morphogenetic protein-induced differentiation of embryonic stem cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 27, pp. 10294-9, (2006) (PubMed).

Chambers: "The molecular basis of pluripotency in mouse embryonic stem cells." in: Cloning and stem cells, Vol. 6, Issue 4, pp. 386-91, (2005) (PubMed).

Chambers, Colby, Robertson, Nichols, Lee, Tweedie, Smith: "Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells." in: Cell, Vol. 113, Issue 5, pp. 643-55, (2003) (PubMed).

Mitsui, Tokuzawa, Itoh, Segawa, Murakami, Takahashi, Maruyama, Maeda, Yamanaka: "The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells." in: Cell, Vol. 113, Issue 5, pp. 631-42, (2003) (PubMed).

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: BUB1B (BUB1B Products)

Background: Full Gene Name: budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Synonyms: BUB1beta,BUBR1,Bub1A,MAD3L,SSK1,hBUBR1

Gene ID: 701