ARF5 antibody (AA 81-180)

Catalog No. ABIN559926

Product Details anti-ARF5 Antibody

Target: ARF5 (ADP-Ribosylation Factor 5 (ARF5))

Binding Specificity: AA 81-180

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARF5 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Purpose: Mouse monoclonal antibody raised against a partial recombinant ARF5.

Sequence: YFQNTQGLIF VVDSNDRERV QESADELQKM LQEDELRDAV LLVFANKQDM PNAMPVSELT DKLGLQHLRS RTWYVQATCA TQGTGLYDGL DWLSHELSKR

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: ARF5 (AAH03043, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 1B4

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-ARF5 antibody (ABIN559926)

Münzberg, Höhn, Krndija, Maaß, Bartsch, Slater, Oswald, Walther, Seufferlein, von Wichert: "IGF-1 drives chromogranin A secretion via activation of Arf1 in human neuroendocrine tumour cells." in: Journal of cellular and molecular medicine, Vol. 19, Issue 5, pp. 948-59, (2015) (PubMed).

Nakai, Kondo, Saitoh, Naito, Nakayama, Shin: "ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus." in: Molecular biology of the cell, Vol. 24, Issue 16, pp. 2570-81, (2013) (PubMed).

Reiling, Olive, Sanyal, Carette, Brummelkamp, Ploegh, Starnbach, Sabatini: "A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens." in: Nature cell biology, Vol. 15, Issue 12, pp. 1473-85, (2013) (PubMed).

Kudelko, Brault, Kwok, Li, Pardigon, Peiris, Bruzzone, Desprès, Nal, Wang: "Class II ADP-ribosylation factors are required for efficient secretion of dengue viruses." in: The Journal of biological chemistry, Vol. 287, Issue 1, pp. 767-77, (2012) (PubMed).

Takashima, Saitoh, Hirose, Nakai, Kondo, Takasu, Kakeya, Shin, Nakayama: "GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER transport involved in regulation of lipid homeostasis." in: Cell structure and function, Vol. 36, Issue 2, pp. 223-35, (2011) (PubMed).

Puxeddu, Uhart, Li, Ahmad, Pacheco-Rodriguez, Manganiello, Moss, Vaughan: "Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 15, pp. 6158-63, (2009) (PubMed).

Klein, Partisani, Franco, Luton: "EFA6 facilitates the assembly of the tight junction by coordinating an Arf6-dependent and -independent pathway." in: The Journal of biological chemistry, Vol. 283, Issue 44, pp. 30129-38, (2008) (PubMed).

Islam, Shen, Hiroi, Moss, Vaughan, Levine: "The brefeldin A-inhibited guanine nucleotide-exchange protein, BIG2, regulates the constitutive release of TNFR1 exosome-like vesicles." in: The Journal of biological chemistry, Vol. 282, Issue 13, pp. 9591-9, (2007) (PubMed).

Target Details for ARF5

Target: ARF5 (ADP-Ribosylation Factor 5 (ARF5))

Alternative Name: ARF5 (ARF5 Products)

Synonyms: ARF/CPS3 antibody, ADP ribosylation factor 5 antibody, ADP-ribosylation factor 5 antibody, ARF5 antibody, Arf5 antibody

Background: Full Gene Name: ADP-ribosylation factor 5
Synonyms:

Gene ID: 381