beta Actin antibody (AA 1-375)

Catalog No. ABIN559756

Product Details anti-beta Actin Antibody

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Binding Specificity: AA 1-375

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Mouse monoclonal antibody raised against a full length recombinant ACTB.

Sequence: MDDDIAALVV DNGSGMCKAG FAGDDAPRAV SPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERRYSVW IGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF

Cross-Reactivity: Human, Mouse

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: ACTB (AAH01301, 1 a.a. ~ 375 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 3G4-F9

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-beta Actin antibody (ABIN559756)

Beard, Zheng, Lagisetty, Burns, Tran, Hewitt, Abate-Daga, Rosati, Fine, Ferrone, Rosenberg, Morgan: "Multiple chimeric antigen receptors successfully target chondroitin sulfate proteoglycan 4 in several different cancer histologies and cancer stem cells." in: Journal for immunotherapy of cancer, Vol. 2, pp. 25, (2014) (PubMed).

Cheng, Yang, Yang, Yang, Deng, Yu, Huang, Zhang, Wang, Qiu, Zhou, Lu: "A microRNA-135a/b binding polymorphism in CD133 confers decreased risk and favorable prognosis of lung cancer in Chinese by reducing CD133 expression." in: Carcinogenesis, Vol. 34, Issue 10, pp. 2292-9, (2013) (PubMed).

Yu, Shen, Yu, Zhong, Zhang, Zhang, Zhao, Li, Chen, Liu, Yang: "Systematic proteomic analysis of human hepotacellular carcinoma cells reveals molecular pathways and networks involved in metastasis." in: Molecular bioSystems, Vol. 7, Issue 6, pp. 1908-16, (2011) (PubMed).

Yamazaki, Sumikura, Hidaka, Yasui, Kiso, Yakushiji, Hayashi: "Anti-microtubule 'plinabulin' chemical probe KPU-244-B3 labeled both alpha- and beta-tubulin." in: Bioorganic & medicinal chemistry, Vol. 18, Issue 9, pp. 3169-74, (2010) (PubMed).

Lin, Chen, Chen, Liou, Chen, Yang, Shiue: "Identification of early transcripts related to male development in chicken embryos." in: Theriogenology, Vol. 74, Issue 7, pp. 1161-1178.e1-8, (2010) (PubMed).

Le Mée, Hennebert, Ferrec, Wülfert, Morfin: "7beta-Hydroxy-epiandrosterone-mediated regulation of the prostaglandin synthesis pathway in human peripheral blood monocytes." in: Steroids, Vol. 73, Issue 11, pp. 1148-59, (2008) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB (ACTB Products)

Background: Full Gene Name: actin, beta
Synonyms: PS1TP5BP1

Gene ID: 60

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction