Histone H4 antibody (N-Term)

Catalog No. ABIN5596994

Product Details anti-Histone H4 Antibody

Target: Histone H4

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity with other species was not tested.

Purification: Anti-Histone H4 pan Antibody antiserum is directed against Histone H4.

Immunogen:

Immunogen: Anti-Histone H4 pan Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the N-terminal region of histone H4.

Immunogen Type: Peptide

Application Details

Application Notes:

Application Note: Anti-Histone H4 pan Antibody is suitable for Western Blot, Chromatin Immunoprecipitation, Dot Blots, and ELISA. Expect a single band approximately 11.3 kDa in size corresponding to H4 by western blot in the appropriate tissue or cell lysate. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500-1:2000

ELISA Dilution: 1:20000

Comment:

Dot Blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Histone H4

Target: Histone H4

Abstract: Histone H4 Products

Synonyms: hist1h4l antibody, H1ft antibody, Hist4 antibody, Hist4h4 antibody, H4 antibody, X04652 antibody, FO108 antibody, H4/n antibody, H4F2 antibody, H4FN antibody, HIST2H4 antibody, H4C13 antibody, H4C7 antibody, F8N16.2 antibody, F8N16_2 antibody, histone H4 antibody, BcDNA:RH52884 antibody, CG3379 antibody, Dmel\CG3379 antibody, H4r antibody, His4-88CD antibody, His4R antibody, histone cluster 1, H4a L homeolog antibody, histone cluster 1 H4 family member B antibody, histone cluster 2, H4 antibody, histone cluster 2 H4 family member a antibody, histone H4 antibody, Histone H4 replacement antibody, Histone H4 antibody, hist1h4a.L antibody, Hist1h4b antibody, Hist2h4 antibody, HIST2H4A antibody, H4 antibody, H4C14 antibody, HIS4 antibody, HIST2H4 antibody, His4r antibody, his-38 antibody

Background:

Synonyms: Histone H4, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4J, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4

Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H4 pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.

Gene Name: HIST1H4A

Gene ID: 121504

UniProt: P62805