WECC antibody (HRP)

Catalog No. ABIN5596986

Product Details anti-WECC Antibody (HRP)

Target: WECC (WecC Gene Product (WECC))

Reactivity: Pseudomonas, Bacteria

Host: Goat

Clonality: Polyclonal

Conjugate: This WECC antibody is conjugated to HRP

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Purification: N-Acylmannosamine-1-Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, and purified and partially purified N-Acylmannosamine-1-Dehydrogenase derived from Pseudomonas.

Immunogen:

Immunogen: N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-N-Acylmannosamine-1-Dehydrogenase has been assayed against 1.0 μg of N-Acylmannosamine-1-Dehydrogenase IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for WECC

Target: WECC (WecC Gene Product (WECC))

Alternative Name: N-Acylmannosamine-1-Dehydrogenase (WECC Products)

Background:

Synonyms: N-acylmannosamine 1-dehydrogenase NAM-DH EC=1.1.1.233

Background: N-Acylmannosamine-1-Dehydrogenase acts on acetyl-D-mannosamine and glycolyl-D-mannosamine. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.

Gene Name: NAM-DH

Gene ID: 7614553

UniProt: P22441