Urate Oxidase antibody (HRP)

Catalog No. ABIN5596984

Product Details anti-Urate Oxidase Antibody (HRP)

Target: Urate Oxidase (UOX)

Reactivity: Bacillus

Host: Goat

Clonality: Polyclonal

Conjugate: This Urate Oxidase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Uricase from other sources is unknown.

Purification: Anti-Uricase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species].

Immunogen:

Immunogen: Anti-uricase Antibody was produced by repeated immunizations with bacillus uricase protein.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Uricase antibody is suitable for western blotting, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Urate Oxidase

Target: Urate Oxidase (UOX)

Alternative Name: Uricase (UOX Products)

Synonyms: AI663847 antibody, UOX-2 antibody, Uri antibody, Uri2 antibody, UOX antibody, urate oxidase antibody, uricase antibody, UOX antibody, Uox antibody, LOC100009266 antibody

Background:

Synonyms: Urate oxidase antibody

Background: Anti-Uricase antibody detects uricase. Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localised in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form, and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation. Anti-Uricase antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.

Gene Name: ABC3735

Gene ID: 3202700

UniProt: Q5WBJ3