TET2 antibody (N-Term)

Catalog No. ABIN5596979

Product Details anti-TET2 Antibody

Target: TET2 (Tet Methylcytosine Dioxygenase 2 (TET2))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TET2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): It is directed against, and shows specific reactivity for human Tet2 protein. Cross reactivity with Tet1 and Tet3 has not been determined.

Purification: Anti-TET2 Antibody was prepared from whole rabbit antiserum by delipidation and defibrination. The antiserum was further cross-absorbed against MBP by chromatography.

Immunogen:

Immunogen: TET2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a human TET-2 domain containing the N-terminal 156 amino acids of the protein.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Application Note: Tet2 antibody has been tested for use by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect band at ~230 kDa and ~150 kDa (isoforms).

Western Blot Dilution: 1:5000

ELISA Dilution: 1:50,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 72.50 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TET2

Target: TET2 (Tet Methylcytosine Dioxygenase 2 (TET2))

Alternative Name: TET2 (TET2 Products)

Synonyms: KIAA1546 antibody, MDS antibody, Ayu17-449 antibody, E130014J05Rik antibody, mKIAA1546 antibody, RGD1311625 antibody, tet methylcytosine dioxygenase 2 antibody, TET2 antibody, Tet2 antibody

Background:

Synonyms: Tet2 antibody, Methylcytosine dioxygenase TET2, KIAA1546 antibody, myeloproliferative disorder antibody, MPD antibody, hematopoietic cell antibody, TET2 antibody,Tet oncogene 2 antibody, MDS antibody, TET2_HUMAN antibody

Background: Tet2 antibody is a dioxygenase enzyme which is associated with the addition of the hydroxyl group to 5-methylcystosine (5mC) to form 5-hydroxymethylcystosine. Tet2 mediates the formation of 5-formylcystosine from 5hmC and subsequently 5fC to 5-carboxylcystosine, and consequently Tet2 plays an active role in catalyzing those conversions which have been suggested as the first step for active mammalian DNA de-methylation. Tet2 enzyme aids in the epigenetic modification of mammalian cystosine bases, ultimately affecting transcriptional regulation. Additionally methylcystosine dioxygenase Tet2 recruits O-GlcNAc transferase OGT enzyme to CpG-rich transcription start sites of the gene, encouraging H2B GlcNAcylation by OGT. Anti-TET2 antibodies are ideal for researchers interested in Epigenetics, Cancer, Chromatin Research and Histone research.

Gene Name: TET2

Gene ID: 54790

UniProt: Q6N021

Pathways: Warburg Effect