Protein G antibody (Biotin)

Catalog No. ABIN5596972

Product Details anti-Protein G Antibody (Biotin)

Target: Protein G

Reactivity: Streptococcus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Protein G antibody is conjugated to Biotin

Application: ELISA

Cross-Reactivity (Details): Cross reactivity against Protein G from other sources may occur but have not been specifically determined.

Purification: Anti-Protein G is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Protein G [Streptococcus species].

Immunogen:

Immunogen: Protein G [Streptococcus species]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Protein G Biotin Conjugated Antibody has been assayed against 1.0 μg of Protein G in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:90,000 to 1:350,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:90,000-1:350,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Protein G

Target: Protein G

Abstract: Protein G Products

Background:

Synonyms: BAC, Protein G Biotin, Anti-Protein G BAC Antibody

Background: Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Protein G antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.