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Protein A antibody (HRP)

Reactivity: Staphylococcus aureus ELISA, WB, IHC Host: Goat Polyclonal HRP
Catalog No. ABIN5596970
  • Target See all Protein A Antibodies
    Protein A
    Reactivity
    • 50
    • 5
    • 1
    Staphylococcus aureus
    Host
    • 20
    • 15
    • 14
    • 5
    • 2
    Goat
    Clonality
    • 49
    • 2
    Polyclonal
    Conjugate
    • 11
    • 8
    • 8
    • 7
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Protein A antibody is conjugated to HRP
    Application
    • 36
    • 34
    • 13
    • 13
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
    Purification
    Anti-Protein A (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus].
    Immunogen

    Immunogen: Protein A [Staphylococcus aureus]

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product Protein A Primary Antibody
  • Application Notes

    Immunohistochemistry Dilution: 1:250

    Application Note: Anti-Protein A Peroxidase Conjugated has been assayed against 1.0 μg of Protein A [Staphylococcus aureus] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 is suggested for this product.

    Western Blot Dilution: 1:500-1:2,000

    ELISA Dilution: 1:2,000-1:20,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    Protein A
    Abstract
    Protein A Products
    Synonyms
    alpha protein antibody, repB antibody
    Background

    Synonyms: HRP, Horseradish Peroxidase, Anti-Protein A HRP Antibody

    Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunglobulins. Horse Raddish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Protein A (GOAT) Antibody is ideal for researchers in Immunology, Cancer, and Microbiology.

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