R-Phycoerythrin antibody (Rhodamine)

Catalog No. ABIN5596967

Product Details anti-R-Phycoerythrin Antibody (Rhodamine)

Target: R-Phycoerythrin (Phycoerythrin, R-)

Host: Goat

Clonality: Polyclonal

Conjugate: This R-Phycoerythrin antibody is conjugated to Rhodamine

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Cross-Reactivity (Details): This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG.

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 20

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular weight fragments is typically noted by western blot.

IF Microscopy Dilution: 1:500-1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for R-Phycoerythrin

Target: R-Phycoerythrin (Phycoerythrin, R-)

Alternative Name: R-Phycoerythrin (R-Phycoerythrin Products)

Target Type: Chemical

Background:

Synonyms: TRITC

Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.