R-Phycoerythrin antibody (HRP)

Catalog No. ABIN5596966

Supplier Product No.: 600-103-387

Product Details anti-R-Phycoerythrin Antibody (HRP)

Target: R-Phycoerythrin (Phycoerythrin, R-)

Host: Goat

Clonality: Polyclonal

Conjugate: This R-Phycoerythrin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-103-387

Supplier: Rockland

Purpose: R-Phycoerythrin Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Goat Serum and R-Phycoerythrin conjugated IgG.

Characteristics: Synonyms: goat anti-R-Phycoerythrin Antibody peroxidase Conjugation, HRP conjugated goat anti-R-Phycoerythrin Antibody

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:300,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for R-Phycoerythrin

Target: R-Phycoerythrin (Phycoerythrin, R-)

Alternative Name: R-Phycoerythrin (R-Phycoerythrin Products)

Target Type: Chemical

Background: Background: Anti-R-Phycoerythrin Antibody Peroxidase Conjugated generated in goat recognizes RPE. Phycoerythrin (PE) is a red protein-pigment present in red algae and cryptophytes and an accessory pigment to the main chlorophyll pigments responsible for photosynthesis. R-phycoerythrin is predominantly produced by red algae. The molecular weight of R-PE is 240 kDa. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-R-PE antibody is conjugated to HRP.