R-Phycoerythrin antibody (FITC)

Catalog No. ABIN5596965

Supplier Product No.: 600-102-387

Product Details anti-R-Phycoerythrin Antibody (FITC)

Target: R-Phycoerythrin (Phycoerythrin, R-)

Host: Goat

Clonality: Polyclonal

Conjugate: This R-Phycoerythrin antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Supplier Product No.: 600-102-387

Supplier: Rockland

Purpose: R-Phycoerythrin Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum and R-Phycoerythrin conjugated IgG.

Characteristics: Synonyms: goat anti-R-Phycoerythrin Antibody fluorescein Conjugation, FITC conjugated goat anti-R-Phycoerythrin Antibody

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 3.0

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Application Note: This product is designed for FACS, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

IF Microscopy Dilution: 1:500-1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for R-Phycoerythrin

Target: R-Phycoerythrin (Phycoerythrin, R-)

Alternative Name: R-Phycoerythrin (R-Phycoerythrin Products)

Target Type: Chemical

Background: Background: Anti-R-Phycoerythrin Antibody Fluorescein Conjugated generated in goat recognizes RPE. Phycoerythrin (PE) is a red protein-pigment present in red algae and cryptophytes and an accessory pigment to the main chlorophyll pigments responsible for photosynthesis. R-phycoerythrin is predominantly produced by red algae. The molecular weight of R-PE is 250 kDa. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-R-PE antibody is conjugated to FITC.