Maltose Phosphorylase antibody (HRP)
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- Target See all Maltose Phosphorylase products
- Maltose Phosphorylase
- Reactivity
- E. coli, Bacteria
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Maltose Phosphorylase antibody is conjugated to HRP
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Application
- Western Blotting (WB), Immunoprecipitation (IP), ELISA
- Cross-Reactivity (Details)
- Cross reactivity against Maltose Phosphorylase from other sources is unknown.
- Purification
- Maltose Phosphorylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Maltose Phosphorylase [E.coli].
- Immunogen
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Immunogen: Maltose Phosphorylase [E.coli]
Immunogen Type: Native Protein
- Isotype
- IgG
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anti-Maltose Phosphorylase antibody (Biotin)
Reactivity: E. coli WB, IP, ELISA Host: Goat Polyclonal Biotin
anti-Maltose Phosphorylase antibodyReactivity: E. coli Host: Goat Polyclonal unconjugated
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- Application Notes
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Application Note: Anti-Maltose Phosphorylase has a working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Maltose Phosphorylase
- Abstract
- Maltose Phosphorylase Products
- Background
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Synonyms: mapA antibody, mpA antibody
Background: Maltose Phosphorylase belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is maltose:phosphate 1-beta-D-glucosyltransferase. This enzyme participates in starch and sucrose metabolism. Two substrates of this enzyme are maltose and phosphate, whereas its two products are D-glucose and beta-D-glucose 1-phosphate.
Gene Name: LAF
- Gene ID
- 6233598
- UniProt
- B2GEX2
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