Malate Dehydrogenase (MDH) antibody (HRP)

Catalog No. ABIN5596953

Product Details

Target: Malate Dehydrogenase (MDH)

Reactivity: Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Malate Dehydrogenase from other sources may occur but has not been specifically determined.

Purification: Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].

Immunogen:

Immunogen: Malate Dehydrogenase [Pig Heart]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Malate Dehydrogenase is useful in ELISA, western blot, and immunohistochemistry. Optimal titers should be obtained by researchers.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:50,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Malate Dehydrogenase (MDH)

Alternative Name: Malate Dehydrogenase (MDH Products)

Synonyms: CG5889 antibody, Dmel\\CG5889 antibody, MDH antibody, Mdh antibody, anon-EST:Posey79 antibody, MALATE DEHYDROGENASE antibody, malate dehydrogenase antibody, An15g00070 antibody, AO090701000013 antibody, DDBDRAFT_0188779 antibody, DDBDRAFT_0230186 antibody, DDB_0188779 antibody, DDB_0230186 antibody, Malic enzyme b antibody, oxidoreductase, putative antibody, malate dehydrogenase antibody, NAD dependent malate dehydrogenase Mdh antibody, Men-b antibody, malate dehydrogenase antibody, MDH antibody, mdh antibody, APH_RS02635 antibody, ANI_1_12134 antibody, AOR_1_26114 antibody, PMI_RS16925 antibody, mdhA antibody, mdh1 antibody

Background:

Synonyms: Malate dehydrogenase, cytoplasmic EC=1.1.1.37 Cytosolic malate dehydrogenase

Background: Malate Dehydrogenase is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate using the reduction of NAD+ to NADH. Malate Dehydrogenase is involved in gluconeogenesis, the synthesis of glucose from smaller molecules. There are two main isoforms in eukaryotic cells. One is found in the mitochondrial matrix the other is found in the cytoplasm.

Gene Name: MDH1

Gene ID: 396894

UniProt: P11708

Pathways: Regulation of Lipid Metabolism by PPARalpha