Luciferase antibody (HRP)

Catalog No. ABIN5596940

Product Details anti-Luciferase Antibody (HRP)

Target: Luciferase

Reactivity: Firefly

Host: Goat

Clonality: Polyclonal

Conjugate: This Luciferase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)].

Immunogen:

Immunogen: Luciferase [Photinus pyralis (Firefly)]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This product has been assayed against 1.0 μg of Luciferase [Photinus pyralis (Firefly)] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:200,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:2,000 - 1:30,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Luciferase antibody (HRP) (ABIN5596940)

Windahl, Lagerquist, Andersson, Jochems, Kallkopf, Håkansson, Inzunza, Gustafsson, van der Saag, Carlsten, Pettersson, Ohlsson: "Identification of target cells for the genomic effects of estrogens in bone." in: Endocrinology, Vol. 148, Issue 12, pp. 5688-95, (2008) (PubMed).

Target Details for Luciferase

Target: Luciferase

Abstract: Luciferase Products

Synonyms: hypothetical protein antibody, luciferase antibody, C25G6.3 antibody, Gbro_2186 antibody

Background:

Synonyms: HRP, Luciferase Peroxidase, Anti-Luciferase HRP Antibody

Background: Anti-Luciferase Peroxidase conjugated antibody is produced from Photinus pyralis (Firefly) which produces a green light with a wavelength of 562 nm. Expect a band at approximately 60.7 kDa in size corresponding to Luciferase by western blotting in the appropriate cell lysate or extract.