Luciferase antibody (FITC)

Catalog No. ABIN5596939

Product Details anti-Luciferase Antibody (FITC)

Target: Luciferase

Reactivity: Firefly

Host: Goat

Clonality: Polyclonal

Conjugate: This Luciferase antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Purification: Anti-Luciferase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum and purified and partially purified Luciferase (Photinus pyralis (Firefly)).

Immunogen:

Immunogen: Luciferase (Photinus pyralis (Firefly))

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 5.6

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Luciferase antibody (FITC) (ABIN5596939)

Windahl, Lagerquist, Andersson, Jochems, Kallkopf, Håkansson, Inzunza, Gustafsson, van der Saag, Carlsten, Pettersson, Ohlsson: "Identification of target cells for the genomic effects of estrogens in bone." in: Endocrinology, Vol. 148, Issue 12, pp. 5688-95, (2008) (PubMed).

Target Details for Luciferase

Target: Luciferase

Abstract: Luciferase Products

Synonyms: hypothetical protein antibody, luciferase antibody, C25G6.3 antibody, Gbro_2186 antibody

Background:

Synonyms: FITC, Luciferase FITC, Anti-Luciferase Fluorescein Antibody

Background: Anti-Luciferase Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.