Lactate Dehydrogenase A antibody (HRP)

Catalog No. ABIN5596934

Supplier Product No.: 200-1373-0100

Product Details anti-Lactate Dehydrogenase A Antibody (HRP)

Target: Lactate Dehydrogenase A (LDHA)

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This Lactate Dehydrogenase A antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Supplier Product No.: 200-1373-0100

Supplier: Rockland

Purpose: Lactate Dehydrogenase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].

Characteristics: Synonyms: goat anti-Lactate Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M

Purification: Anti-Lactate Dehydrogenase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Lactate Dehydrogenase Antibody was produced by repeated immunizations with rabbit muscle Lactate Dehydrogenase full length protein.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Lactate Dehydrogenase peroxidase Antibody has been tested in ELISA, western blot, and immunofluorescence. This product is suitable for IP and IHC. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1,000 - 1:6,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:3,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Lactate Dehydrogenase A

Target: Lactate Dehydrogenase A (LDHA)

Alternative Name: LDHA (LDHA Products)

Background: Background: Anti-Lactate Dehydrogenase Antibody detects Lactate Dehydrogenase. Lactate dehydrogenases exist in four distinct enzyme classes. Two of them are cytochrome c-dependent enzymes, each acting on either D-lactate or L-lactate. The other two are NAD(P)-dependent enzymes, each acting on either D-lactate or L-lactate. Lactate dehydrogenase catalyzes the interconversion of pyruvate and lactate. Anti-Lactate Dehydrogenase Antibody is ideal for investigators involved in Cell Signaling, Immunology, Neuroscience and Signal Transduction research.

Gene ID: 100009107, 126050

UniProt: P13491

Pathways: Warburg Effect