EDC3 antibody (Internal Region, pSer161)

Catalog No. ABIN5596933

Product Details anti-EDC3 Antibody

Target: EDC3 (Enhancer of mRNA Decapping 3 (EDC3))

Binding Specificity: Internal Region, pSer161

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EDC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with rabbit, pig, monkey, bovine, rat, mouse, and bat based on 100% sequence homology. Cross-reactivity with EDC3 pS161 from other sources has not been determined.

Purification: Anti-EDC3 pS161 is specific for phospho S161 mouse EDC3. It was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Immunogen:

Immunogen: EDC3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide surrounding the pS161 site of mouse EDC3.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-EDC3pS161 antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~55 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 0.5 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for EDC3

Target: EDC3 (Enhancer of mRNA Decapping 3 (EDC3))

Alternative Name: EDC3 (EDC3 Products)

Background:

Synonyms: Enhancer of mRNA-decapping protein 3, YjeF domain-containing protein 1, Edc3, Yjdc

Background: EDC3 antibody is associated with an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end. It has also been implicated in cell signaling involving the 14-3-3 proteins and related pathways. Anti-EDC3 [pS161] antibody is ideal for investigators interested in Epigenetic and Nuclear Signaling research.

Gene Name: Edc3