CD2AP antibody (pTyr4, pTyr8, pTyr10)

Catalog No. ABIN5596919

Product Details anti-CD2AP Antibody

Target: CD2AP (Cd2ap) (CD2-Associated Protein (Cd2ap))

Binding Specificity: pTyr4, pTyr8, pTyr10

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD2AP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: CD2AP phospho Y4-8-10 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with human based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-CD2AP pY 4-8-10 Antibody, Adapter protein CMS, CD2AP, CD2-associated protein, Cas ligand with multiple SH3 domains, rabbit anti-CD2AP phosphorylated antibody

Purification: Anti-CD2AP pY4-8-10 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-CD2AP pY 4-8-10 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide at Tyr-4-8-10 at the N-terminal of human CD2AP protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-CD2AP antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~71 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.04 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol with 1 mg/mL Bovine Serum Albumin (BSA)

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CD2AP

Target: CD2AP (Cd2ap) (CD2-Associated Protein (Cd2ap))

Alternative Name: CD2AP (Cd2ap Products)

Background: Background: CD2-associated protein appears to act as an adapter protein between membrane proteins and the actin cytoskeleton. It may also be a factor in receptor clustering and cytoskeletal polarity between T-cell and antigen-presenting cell. It seems it could also anchor the podocyte slit diaphragm to the actin skeleton in renal glomerulus. It is required for cytokinesis. CD2AP is widely expressed in fetal and adult tissue, localized in the cytoplasm of cells. Anti-CD2AP pY4-8-10 Antibody is ideal for investigators interested in Signal Transduction research.

UniProt: Q9Y5K6