Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Trypsin antibody (HRP)

Reactivity: Cow WB, ELISA, IHC Host: Rabbit Polyclonal HRP
Catalog No. ABIN5596916
  • Target See all Trypsin Antibodies
    Trypsin
    Reactivity
    • 12
    • 11
    • 9
    Cow
    Host
    • 27
    • 5
    Rabbit
    Clonality
    • 25
    • 7
    Polyclonal
    Conjugate
    • 21
    • 7
    • 2
    • 2
    This Trypsin antibody is conjugated to HRP
    Application
    • 25
    • 24
    • 8
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Cross-Reactivity (Details)
    Cross reactivity against Trypsin from other sources is unknown.
    Purification
    Trypsin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin [Bovine Pancreas].
    Immunogen

    Immunogen: Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product Trypsin Primary Antibody
  • Application Notes

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-Trypsin Antibody is suitable for western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

    Western Blot Dilution: 1:1,000 - 1:5,000

    ELISA Dilution: 1:14,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    Trypsin
    Abstract
    Trypsin Products
    Synonyms
    wu:fb57g08 antibody, zgc:109701 antibody, LOC397853 antibody, Trypsin antibody, trp1 antibody, try1 antibody, trypsin antibody, protease, serine 1 antibody, protease, serine 1 L homeolog antibody, protease, serine, 1 antibody, trypsin antibody, prss1 antibody, prss1.L antibody, Deipr_2253 antibody
    Background

    Synonyms: Anionic trypsinogen antibody, Beta trypsin antibody, Brain trypsinogen antibody, Cationic Trypsinogen antibody, Digestive Zymogen antibody, Mesotrypsin antibody, Mesotrypsinogen antibody

    Background: Anti-Trypsin Antibody detects trypsin. Trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. It belongs to the peptidase S1 family. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. It cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. Trypsin hydrolyzes ester and amide linkages of several synthetic substrates. Anti-Trypsin Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction and Biochemistry research.

    Gene Name: TRBOTR

    Gene ID
    780933
    UniProt
    P00760
You are here:
Support