Trypsin antibody (HRP)
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- Target See all Trypsin Antibodies
- Trypsin
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Reactivity
- Cow
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Trypsin antibody is conjugated to HRP
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Cross-Reactivity (Details)
- Cross reactivity against Trypsin from other sources is unknown.
- Purification
- Trypsin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin [Bovine Pancreas].
- Immunogen
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Immunogen: Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.
Immunogen Type: Native Protein
- Isotype
- IgG
- Top Product
- Discover our top product Trypsin Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Trypsin Antibody is suitable for western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:14,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Trypsin
- Abstract
- Trypsin Products
- Synonyms
- wu:fb57g08 antibody, zgc:109701 antibody, LOC397853 antibody, Trypsin antibody, trp1 antibody, try1 antibody, trypsin antibody, protease, serine 1 antibody, protease, serine 1 L homeolog antibody, protease, serine, 1 antibody, trypsin antibody, prss1 antibody, prss1.L antibody, Deipr_2253 antibody
- Background
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Synonyms: Anionic trypsinogen antibody, Beta trypsin antibody, Brain trypsinogen antibody, Cationic Trypsinogen antibody, Digestive Zymogen antibody, Mesotrypsin antibody, Mesotrypsinogen antibody
Background: Anti-Trypsin Antibody detects trypsin. Trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. It belongs to the peptidase S1 family. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. It cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. Trypsin hydrolyzes ester and amide linkages of several synthetic substrates. Anti-Trypsin Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction and Biochemistry research.
Gene Name: TRBOTR
- Gene ID
- 780933
- UniProt
- P00760
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