CPB1 antibody (HRP)

Catalog No. ABIN5596913

Product Details anti-CPB1 Antibody (HRP)

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Reactivity: Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPB1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Carboxypeptidase B from other tissues and species is unknown.

Purification: Anti-Carboxypeptidase B Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas].

Immunogen:

Immunogen: Carboxypeptidase B [Porcine Pancreas]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Carboxypeptidase B Antibody has been assayed against 1.0 μg of Carboxypeptidase B [Porcine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for Anti-Carboxypeptidase B Antibody.

Western Blot Dilution: 1:250 - 1:1,000

ELISA Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CPB1

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Alternative Name: Carboxypeptidase B (CPB1 Products)

Background:

Synonyms: CarboxypeptidaseB1 antibody, CP B1 antibody, CPB 1 antibody, CPB antibody, CPB1 antibody, DKFZp779K1333 antibody, Pancreas specific protein antibody, Pancreatic carboxypeptidase B1 antibody

Background: Anti-Carboxypeptidase B Antibody is a key enzyme in the hydrolysis in basic amino acids in the C-terminal position of polypeptides. First isolated from the porcine pancreas, Carboxypeptidase B is activated by trypsin and exhibits high specificity for lysine and arginine. Carboxypeptidase B is also a key enzyme in insulin product in humans and can be used as a marker for pancreatic diagnosis. Anti-Carboxypeptidase B is ideal for investigators in Cell Biology, Immunology, and Microbiology.

Gene Name: CPB1

Gene ID: 397341

UniProt: P09955