CPB1 antibody (HRP)

Catalog No. ABIN5596913

Product Details anti-CPB1 Antibody (HRP)

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Reactivity: Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPB1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Carboxypeptidase B from other tissues and species is unknown.

Purification: Anti-Carboxypeptidase B Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas].

Immunogen:

Immunogen: Carboxypeptidase B [Porcine Pancreas]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Carboxypeptidase B Antibody has been assayed against 1.0 μg of Carboxypeptidase B [Porcine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for Anti-Carboxypeptidase B Antibody.

Western Blot Dilution: 1:250 - 1:1,000

ELISA Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CPB1

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Alternative Name: Carboxypeptidase B (CPB1 Products)

Synonyms: wu:fb60d07 antibody, wu:fb69b08 antibody, CPB1 antibody, pCPB antibody, CPB antibody, PASP antibody, PCPB antibody, Cpb antibody, 0910001A18Rik antibody, 1810063F02Rik antibody, 2210008M23Rik antibody, AI504870 antibody, carboxypeptidase B1 (tissue) antibody, carboxypeptidase B1 antibody, carboxypeptidase B1 S homeolog antibody, carboxypeptidase B antibody, cpb1 antibody, CPB1 antibody, cpb1.S antibody, CpipJ_CPIJ010799 antibody, CpipJ_CPIJ010801 antibody, VDBG_02663 antibody, LOC100562609 antibody, Cpb1 antibody

Background:

Synonyms: CarboxypeptidaseB1 antibody, CP B1 antibody, CPB 1 antibody, CPB antibody, CPB1 antibody, DKFZp779K1333 antibody, Pancreas specific protein antibody, Pancreatic carboxypeptidase B1 antibody

Background: Anti-Carboxypeptidase B Antibody is a key enzyme in the hydrolysis in basic amino acids in the C-terminal position of polypeptides. First isolated from the porcine pancreas, Carboxypeptidase B is activated by trypsin and exhibits high specificity for lysine and arginine. Carboxypeptidase B is also a key enzyme in insulin product in humans and can be used as a marker for pancreatic diagnosis. Anti-Carboxypeptidase B is ideal for investigators in Cell Biology, Immunology, and Microbiology.

Gene Name: CPB1

Gene ID: 397341

UniProt: P09955