CA2 antibody (HRP)

Catalog No. ABIN5596909

Product Details anti-CA2 Antibody (HRP)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA2 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Cross-Reactivity (Details): Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined.

Purification: Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].

Immunogen:

Immunogen: Carbonic Anhydrase II [Bovine Erythrocytes]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Carbonic anhydrase II has been assayed against 1.0 μg of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: Carbonic Anhydrase II (CA2 Products)

Background:

Synonyms: Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody

Background: Carbonic anhydrase II (CA2), is one of fourteen forms of human α carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

Gene Name: CA2

Gene ID: 280740

UniProt: P00921