Amylase beta antibody (HRP)
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- Target See all Amylase beta (amyB) products
- Amylase beta (amyB)
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Reactivity
- Sweet potato
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Amylase beta antibody is conjugated to HRP
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Application
- Western Blotting (WB), ELISA
- Cross-Reactivity (Details)
- Cross reactivity against Beta Amylase from other tissues and species may occur but have not been specifically determined.
- Purification
- Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Beta Amylase [Sweet Potato].
- Immunogen
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Immunogen: Beta Amylase [Sweet Potato]
Immunogen Type: Native Protein
- Isotype
- IgG
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anti-Amylase beta (amyB) antibody (Biotin)
amyB Reactivity: Sweet potato WB, ELISA Host: Rabbit Polyclonal Biotin
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- Application Notes
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Application Note: Anti-Beta Amylase has been assayed against 1.0 μg of Beta Amylase [Sweet Potato] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:10,000 - 1:40,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Amylase beta (amyB)
- Alternative Name
- Beta Amylase (amyB Products)
- Synonyms
- beta-amylase antibody, Beta-amylase antibody, CBO1203 antibody, amyB antibody, CLM_1356 antibody, BMQ_0356 antibody, BMD_0357 antibody
- Background
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Synonyms: 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody
Background: Beta Amylase acts on starch, glycogen and related polysaccharides and oligosaccharides producing beta-maltose by an inversion.
Gene Name: BMY1, AMYB
- UniProt
- P10537
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