beta-Amylase (BAM1) antibody (HRP)

Catalog No. ABIN5596905

Supplier Product No.: 200-4347-0100

Product Details

Target: beta-Amylase (BAM1)

Reactivity: Sweet potato

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Dot Blot (DB)

Supplier Product No.: 200-4347-0100

Supplier: Rockland

Purpose: Beta Amylase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Beta Amylase [Sweet Potato].

Characteristics: Synonyms: rabbit anti-Beta Amylase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Beta Amylase Antibody, 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody

Purification: Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Beta Amylase [Sweet Potato]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Beta Amylase Peroxidase conjugated antibody has been tested by ELISA and dot blot This product is assayed against 1.0 μg of Beta Amylase [Sweet Potato] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:1,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:250

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: beta-Amylase (BAM1)

Alternative Name: BMY1, AMYB

Background: Background: Beta Amylase acts on starch, glycogen and related polysaccharides and oligosaccharides producing beta-maltose by an inversion.

UniProt: P10537