Alcohol Dehydrogenase (ADH) antibody (HRP)

Catalog No. ABIN5596893

Product Details

Target: Alcohol Dehydrogenase (ADH)

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity against Alcohol Dehydrogenase from other sources is unknown.

Purification: Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast].

Immunogen:

Immunogen: Alcohol Dehydrogenase [Yeast]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Alcohol Dehydrogenase are useful for ELISA, western blot, and immunohistochemistry. Optimal titiers are to be optimized bt researchers.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:4,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Alcohol Dehydrogenase (ADH)

Alternative Name: Alcohol Dehydrogenase (ADH Products)

Background:

Synonyms: Alcohol dehydrogenase 1 EC=1.1.1.1 Alcohol dehydrogenase I YADH-1

Background: Alcohol Dehydrogenases (ADH) are a group of dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of nicotinamide adenine dinucleotide (NAD+ to NADH). In humans and many other animals, they serve to break down alcohols that otherwise are toxic, and they also participate in generation of useful aldehyde, ketone, or alcohol groups during biosynthesis of various metabolites.

Gene Name: ADH1, ADC1, YOL086C

Gene ID: 2538902

UniProt: P00330