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GFP antibody

Name: GFP antibody
SKU: ABIN1715175
Price: 218.43 USD
Availability: InStock

GFP Reactivity: Aequorea victoria WB, ELISA, IP, DB, ISt Host: Mouse Monoclonal GF28R unconjugated

1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1μg (2) and 10μg (3) of cell lysates used (GFP antibody)

1 image

(1 reference)

Catalog No. ABIN1715175

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
175

15

11

9
Aequorea victoria
Host
64

63

34

13

9

5

2

1
Mouse
Compatible Secondaries
Clonality
113

73
Monoclonal
Conjugate
82

12

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
Application
148

80

47

34

27

25

23

21

14

13

13

12

12

5

4

4

4

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Immunostaining (ISt)

Specificity

Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP

Sensitivity

WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins

Purification

Protein A affinity chromatography from mouse ascites fluid

Immunogen

GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates

Clone

GF28R

Isotype

IgG2b

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Application Notes

WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Restrictions

For Research Use only
Concentration

1mg/mL

Buffer

10mM PBS, pH 7.2, 0.05% NaN3

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C
Chi, Bo, Wu, Jong, Huang: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, (2012) (PubMed).
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody