Glucose Oxidase antibody (HRP)
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- Target See all Glucose Oxidase (GOD) products
- Glucose Oxidase (GOD)
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Reactivity
- Aspergillus niger
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Glucose Oxidase antibody is conjugated to HRP
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Supplier Product No.
- 200-4337-0100
- Supplier
- Rockland
- Purpose
- Glucose Oxidase Antibody Peroxidase Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].
- Characteristics
- Synonyms: rabbit anti-Glucose Oxidase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
- Purification
- Glucose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
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Immunogen: Glucose Oxidase [Aspergillus niger]
Immunogen Type: Native Protein
- Isotype
- IgG
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anti-Glucose Oxidase (GOD) antibody
GOD Reactivity: Aspergillus niger ELISA, WB, IHC Host: Goat Polyclonal unconjugated
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- Application Notes
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Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: Anti-Glucose Oxidase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is useful in Immunohistochemistry. Optimal titers should be obtained by researcher.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Glucose Oxidase (GOD)
- Alternative Name
- Glucose Oxidase (GOD Products)
- Background
- Background: Glucose Oxidase is an oxido-reductase that catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites, used for the determination of free glucose in body fluids, and has many applications in biotechnologies.
- UniProt
- P13006
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