Myosin antibody (N-Term, pSer19, pSer20)

Catalog No. ABIN5596872

Product Details anti-Myosin Antibody

Target: Myosin

Binding Specificity: N-Term, pSer19, pSer20

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myosin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Cross-Reactivity (Details): Anti-Myosin pS19/pS20 antibody is directed against the regulatory light chain of smooth and non-muscle myosin. This antiserum is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein. Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA. Cross reactivity is expected with myosin light chain from human and mouse. Reactivity with the protein from other species has not been determined. However, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.

Immunogen:

Immunogen: Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-Myosin pS19/pS20 phospho specific polyclonal antibody was tested by ELISA, immunohistochemistry and immunoblotting. Immunoblotting was used to show reactivity with unstimulated and stimulated cardiac myocytes. The antibody was also reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional by immunoprecipitation.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 70 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Myosin

Target: Myosin

Abstract: Myosin Products

Synonyms: CG3201 antibody, DmMLC_C antibody, Dmel\\CG3201 antibody, MyoII antibody, mlc-c antibody, nmELC antibody, MYOSIN antibody, myosin 1 antibody, DFNA4 antibody, DFNA4A antibody, MHC16 antibody, MYH17 antibody, NMHC II-C antibody, NMHC-II-C antibody, PNMHH antibody, myosin antibody, MYH1 antibody, MYH2 antibody, MYH3 antibody, MYH4 antibody, MYH8 antibody, adult antibody, embryonic antibody, 2400004E04Rik antibody, II-C antibody, NHMCII antibody, DROMHC antibody, Dm nmII antibody, DmnmII antibody, Dronm-MII antibody, E(br) antibody, MHC antibody, Mhc-c antibody, Mhc-c[1] antibody, Myo antibody, Myo II antibody, NMM antibody, Zip antibody, anon-WO0140519.37 antibody, l(2)02957 antibody, nmMHC antibody, nmy-2 antibody, zpr antibody, DmelCG15792 antibody, CG15792 antibody, Myosin light chain cytoplasmic antibody, hypothetical protein antibody, myosin 1 antibody, myosin heavy chain 9 antibody, myosin heavy chain 14 antibody, myosin, heavy chain 1B, skeletal muscle antibody, myosin, heavy polypeptide 14 antibody, zipper antibody, Mlc-c antibody, T22C1.6 antibody, ATM1 antibody, MYH9 antibody, MYH14 antibody, Myh14 antibody, MYH1B antibody, zip antibody

Background:

Synonyms: Myosin regulatory light chain 12A, Myosin regulatory light chain MRLC3, Myosin regulatory light chain 2, nonsarcomeric, Myosin RLC, MLC-2B,

Background: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. This antibody is specific for the phosphorylated form of myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.

Gene Name: MYL12A

Gene ID: 10627

UniProt: P19105