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- Target See all Esterase products
- Esterase
- Reactivity
- Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Esterase antibody is conjugated to HRP
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Cross-Reactivity (Details)
- Cross reactivity against Esterase from other tissues and species may occur but have not been specifically determined.
- Purification
- Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].
- Immunogen
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Immunogen: Esterase [Porcine Liver]
Immunogen Type: Native Protein
- Isotype
- IgG
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anti-Esterase antibody (Biotin)
Reactivity: Pig ELISA, WB Host: Rabbit Polyclonal Biotin
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- Application Notes
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Esterase has been assayed against 1.0 μg of Esterase [Porcine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:250 - 1:1,000
ELISA Dilution: 1:1,000 - 1:5,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Esterase
- Abstract
- Esterase Products
- Synonyms
- Carboxylic ester hydrolase antibody, T28C12.4 antibody, K11G9.1 antibody, K07C11.4 antibody, F15A8.6 antibody, K11G9.2 antibody, K11G9.3 antibody, T28C12.5 antibody
- Background
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Synonyms: Liver carboxylesterase, EC=3.1.1.1, Proline-beta-naphthylamidase, Retinyl ester hydrolase
Background: Esterase is a hydrolase enzyme that splits esters into an acid and an alcohol in a chemical reaction with water called hydrolysis.
Gene Name: CES3
- Gene ID
- 100626873
- UniProt
- Q29550
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