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COL6 antibody

COL6 Reactivity: Human, Cow, Mammalian WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5596844
  • Target See all COL6 Antibodies
    COL6 (Collagen, Type VI (COL6))
    Reactivity
    • 31
    • 10
    • 10
    • 5
    • 4
    • 1
    • 1
    • 1
    Human, Cow, Mammalian
    Host
    • 21
    • 9
    • 1
    Rabbit
    Clonality
    • 22
    • 9
    Polyclonal
    Conjugate
    • 17
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This COL6 antibody is un-conjugated
    Application
    • 20
    • 18
    • 15
    • 7
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Cross-Reactivity (Details)
    Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
    Purification
    Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
    Immunogen

    Immunogen: Collagen Type VI from human and bovine placenta

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product COL6 Primary Antibody
  • Application Notes

    Immunohistochemistry Dilution: 1:50 - 1:200

    Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.

    Western Blot Dilution: 1:1,000 - 1:10,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:50,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Ida, Hikage, Itoh, Ida, Ohguro: "Prostaglandin F2α agonist-induced suppression of 3T3-L1 cell adipogenesis affects spatial formation of extra-cellular matrix." in: Scientific reports, Vol. 10, Issue 1, pp. 7958, (2020) (PubMed).

  • Target
    COL6 (Collagen, Type VI (COL6))
    Alternative Name
    Collagen Type VI (COL6 Products)
    Background

    Synonyms: CollagenVI antibody, Human mRNA for collagen VI alpha 1 C terminal globular domain antibody

    Background: Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.

    Gene Name: COL6A1

    Gene ID
    1291
    UniProt
    P12109
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