His Tag antibody

Catalog No. ABIN559683

Product Details anti-His Tag Antibody

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Immunostaining (ISt)

Sequence: HHHHHH

Specificity: Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)

Sensitivity: WB (with ECL): 1-2ng of His-tagged fusion proteins

Purification: Protein A affinity chromatography from mouse ascites fluid

Immunogen: HHHHHH (6x His) synthetic peptide conjugated to KLH

Clone: HIS-H8

Isotype: IgG2b

Product Specific Information:

What can the Collagen 4 antibody ABIN5596835 be used for? This polyclonal collagen 4 antibody is unconjugated and has been validated for ELISA, IHC, IP and western blotting. Native collagen type 4 from human and bovine placenta was used as immunogen for the production of the COL4 antibody.

What validation data is available for this Collagen 4 antibody? The antibody has been referenced in 8 Pubmed publications to date and is characterized by high reliability. The references are given below. 9 images show the performance of the product in different applications.

What is the function of Collagen 4? Collagen type 4 is the major structural component of glomerular basement membranes, forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. (Uniprot). The collagen 4 protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. (NCBI)

Application Details

Application Notes: WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Restrictions: For Research Use only

Handling

Concentration: 1mg/mL

Buffer: 10mM PBS, pH 7.2, 0.05% NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-His Tag antibody (ABIN559683)

Fan, Kishore, Jansen-Olliges, Wang, Stahl, Psathaki, Harre, Warnecke, Weder, Preller, Zeilinger: "Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta." in: ACS omega, Vol. 7, Issue 33, pp. 28932-28945, (2022) (PubMed).

Chi, Bo, Wu, Jong, Huang: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, (2012) (PubMed).

Hu, Benedict, Ding, Núñez: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, (1999) (PubMed).

Green, Reed: "Mitochondria and apoptosis." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, (1998) (PubMed).

Srinivasula, Ahmad, Fernandes-Alnemri, Alnemri: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." in: Molecular cell, Vol. 1, Issue 7, pp. 949-57, (1998) (PubMed).

Target Details for His Tag

Target: His Tag

Abstract: His Tag Products

Target Type: Tag