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Aspartate Transaminase antibody (HRP)

Ast Reactivity: Pig ELISA, WB Host: Sheep Polyclonal HRP
Catalog No. ABIN5596816
  • Target See all Aspartate Transaminase (Ast) products
    Aspartate Transaminase (Ast)
    Reactivity
    Pig
    Host
    • 4
    Sheep
    Clonality
    • 4
    Polyclonal
    Conjugate
    • 3
    • 1
    This Aspartate Transaminase antibody is conjugated to HRP
    Application
    ELISA, Western Blotting (WB)
    Cross-Reactivity (Details)
    Cross reactivity against Aspartate Aminotransferase (AST) from other sources may occur but has not been specifically determined.
    Purification
    Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].
    Immunogen

    Immunogen: Aspartate Aminotransferase (AST) [Pig Heart]

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Application Note: Anti-Aspartate Transaminase has been assayed against 1.0 μg of Aspartate Aminotransferase (AST) [Pig Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:1,000 - 1:5,000

    ELISA Dilution: 1:10,000 - 1:40,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    Aspartate Transaminase (Ast)
    Alternative Name
    Aspartate Transaminase (Ast Products)
    Synonyms
    Afu6g02490 antibody, Afu2g09650 antibody, aspartate transaminase antibody, Aspartate transaminase antibody, Aspartate transaminase, putative antibody, aspB antibody, CNM00360 antibody, AFUA_6G02490 antibody, AFUA_2G09650 antibody, AAT_1 antibody, Dd586_1627 antibody, aspB3 antibody, Fbal_1917 antibody, Emin_0749 antibody, CGB_M0340W antibody, Sgly_0435 antibody, Weevi_1216 antibody, Selsp_1595 antibody, Trebr_2143 antibody
    Background

    Synonyms: Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody

    Background: Aspartate Transaminase is an important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. It acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Aspartate Transaminase is found in the liver, heart, skeletal muscle, kidneys, brain, and red blood cells, and it is commonly measured clinically as a marker for liver health.

    Gene Name: GOT1

    Gene ID
    396967
    UniProt
    P00503
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