Acid Phosphatase antibody (HRP)
-
- Target See all Acid Phosphatase (ACP) Antibodies
- Acid Phosphatase (ACP)
-
Reactivity
- Potato
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Acid Phosphatase antibody is conjugated to HRP
-
Application
- Western Blotting (WB), ELISA
- Cross-Reactivity (Details)
- Cross reactivity against Acid Phosphatase from other tissues and species may occur but have not been specifically determined.
- Purification
- Acid Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato].
- Immunogen
-
Immunogen: Acid Phosphatase [Potato]
Immunogen Type: Native Protein
- Isotype
- IgG
-
anti-Acid Phosphatase (ACP) antibody (Biotin)
ACP Reactivity: Potato WB, ELISA Host: Rabbit Polyclonal Biotin
-
- Application Notes
-
Application Note: Anti-Acid Phosphatase is suitable for use in ELISA, western blot, and immunohistochemistry. Optimal titers should be optimized by researcher.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:4,000 - 1:20,000
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
-
- Target
- Acid Phosphatase (ACP)
- Alternative Name
- Acid Phosphatase (ACP Products)
- Synonyms
- Acph antibody, ACP antibody, Acid phosphatase antibody, acid phosphatase antibody, cerebral palsy, ataxic 1 antibody, Dana\Acph antibody, phoC antibody, CPAT1 antibody
- Background
-
Synonyms: Acid phosphatase 1 soluble antibody, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody
Background: Acid Phosphatase is a phosphatase, a type of enzyme, used to free attached phosphate groups from other molecules during digestion. It is basically a phosphomonoesterase. It is stored in lysosomes and functions when these fuse with endosomes, which are acidified while they function, therefore, it has an acid pH optimum. Different forms of acid phosphatase are found in different organs, and their serum levels are used to evaluate the success of the surgical treatment of prostate cancer.
Gene Name: PAP3
- UniProt
- Q6J5M8
-
