Acetylated Lysine antibody (Biotin)

Catalog No. ABIN5596810

Product Details anti-Acetylated Lysine Antibody (Biotin)

Target: Acetylated Lysine

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetylated Lysine antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Reactivity is specific for acetylated lysine.

Purification: Anti-AcK was prepared from monospecific antiserum by immunoaffinity chromatography using acetylated lysine peptide coupled to agarose followed by conjugation to biotin and subsequent purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin.

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with acetylated lysine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody is suitable for use in ELISA and western blotting assays. The antibody reacts specifically with acetylated lysine residues. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Western Blot Dilution: 1:1,000-1:5,000

ELISA Dilution: 1:5,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Acetylated Lysine

Target: Acetylated Lysine

Alternative Name: Lysine Acetylated (Acetylated Lysine Products)

Synonyms: C-K-RAS antibody, CFC2 antibody, K-RAS2A antibody, K-RAS2B antibody, K-RAS4A antibody, K-RAS4B antibody, KI-RAS antibody, KRAS1 antibody, KRAS2 antibody, NS antibody, NS3 antibody, RASK2 antibody, KRAS proto-oncogene, GTPase antibody, KRAS antibody

Target Type: Chemical

Background:

Synonyms: acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody

Background: Acetylation refers to the process of introducing an acetyl group into a compound. Proteins are typically acetylated on lysine residues and this reaction relies on acetyl-coenzyme A as the acetyl group donor. Biotin Conjugated Anti-Acetylated Lysine antibody is useful in Cancer, Cell Biology and Chromatin & Nuclear Signaling research.