Acetylated Lysine antibody

Catalog No. ABIN5596809

Product Details anti-Acetylated Lysine Antibody

Target: Acetylated Lysine

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetylated Lysine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Cross-Reactivity (Details): The antibody reacts specifically with acetylated lysine residues.

Purification: Anti-Lysine Acetylated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using acetylated lysine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.

Immunogen:

Immunogen: Anti-Lysine Acetylated Antibody was prepared from whole rabbit serum produced by repeated immunizations with acetylated lysine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Lysine Acetylated Antibody is suitable for use in ELISA, western blotting, immunofluorescence microscopy, and immunoprecipitation assays. Although not tested, this antibody is likely functional in RIA, flow cytometry, and immunohistochemistry.

Western Blot Dilution: 1:500-1:1,000

Immunoprecipitation Dilution: 10 μg/mg protein sample

ELISA Dilution: 1:1,000-1:2,500

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Acetylated Lysine antibody (ABIN5596809)

Zhou, Huang, Zhang, Zhao, Kim, Tu, Zhang, Nowsheen, Zhu, Deng, Chen, Qin, Luo, Liu, Lou, Mutter, Yuan: "The bromodomain containing protein BRD-9 orchestrates RAD51-RAD54 complex formation and regulates homologous recombination-mediated repair." in: Nature communications, Vol. 11, Issue 1, pp. 2639, (2020) (PubMed).

Lin, Yuan, Pei, Liu, Ann, Lou: "KAP1 Deacetylation by SIRT1 Promotes Non-Homologous End-Joining Repair." in: PLoS ONE, Vol. 10, Issue 4, pp. e0123935, (2016) (PubMed).

Pérez-Luna, Aguasca, Perearnau, Serratosa, Martínez-Balbas, Jesús Pujol, Bachs: "PCAF regulates the stability of the transcriptional regulator and cyclin-dependent kinase inhibitor p27 Kip1." in: Nucleic acids research, Vol. 40, Issue 14, pp. 6520-33, (2012) (PubMed).

Target Details for Acetylated Lysine

Target: Acetylated Lysine

Alternative Name: Lysine Acetylated (Acetylated Lysine Products)

Synonyms: C-K-RAS antibody, CFC2 antibody, K-RAS2A antibody, K-RAS2B antibody, K-RAS4A antibody, K-RAS4B antibody, KI-RAS antibody, KRAS1 antibody, KRAS2 antibody, NS antibody, NS3 antibody, RASK2 antibody, KRAS proto-oncogene, GTPase antibody, KRAS antibody

Target Type: Chemical

Background:

Synonyms: acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody

Background: Acetylation refers to the process of introducing an acetyl group into a compound. Proteins are typically acetylated on lysine residues and this reaction relies on acetyl-coenzyme A as the acetyl group donor. Acetylated Lysine antibody is useful in Cancer, Cell Biology and Chromatin & Nuclear Signaling research.