Desmoplakin antibody (C-Term, pSer2849)

Catalog No. ABIN5596788

Product Details anti-Desmoplakin Antibody

Target: Desmoplakin (DSP)

Binding Specificity: C-Term, pSer2849

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Desmoplakin antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100% sequence homology. Cross-reactivity with Desmoplakin pS2849 from other sources has not been determined.

Purification: Anti-Desmoplakin pS2849 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of the S2849 human residue.

Immunogen:

Immunogen: Anti-Desmoplakin pS2849 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the C-terminus of human Desmoplakin protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Desmoplakin pS2849 antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~331 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.67 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Desmoplakin

Target: Desmoplakin (DSP)

Alternative Name: Desmoplakin (DSP Products)

Background:

Synonyms: 250/210 kDa paraneoplastic pemphigus antigen, DSP, DPI, DPII

Background: Desmoplakin pS2849 is high molecular weight protein of desmosomes. It is a member of the plakin or cytolinker family and is involved in the organization of the desmosomal cadherin-plakoglobin complexes into plasma membrane domains in the anchoring of intermediate filaments to the desmosomes. The phosphorylation theoretically affects its association with epidermal, simple cytokeratins and VIM intermediate filaments. Defects in DSP are the cause of keratosis palmoplantaris striata II, Carvajal syndrome, and arrhythmogenic right ventricular cardiomyopathy 8. Anti-Desmoplakin pS2849 Antibody is ideal for researchers interested in Cell Structure research.

Gene Name: DSP

Pathways: Cell-Cell Junction Organization