Cholesterol Oxidase antibody (HRP)

Catalog No. ABIN5596764

Product Details anti-Cholesterol Oxidase Antibody (HRP)

Target: Cholesterol Oxidase (choS)

Reactivity: Streptomyces

Host: Goat

Clonality: Polyclonal

Conjugate: This Cholesterol Oxidase antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Cholesterol Oxidase from other sources is unknown.

Purification: Cholesterol Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism].

Immunogen:

Immunogen: Cholesterol Oxidase [Streptomyces]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Cholesterol Oxidase has been assayed against 1.0 μg of Cholesterol Oxidase [Microorganism] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Cholesterol Oxidase

Target: Cholesterol Oxidase (choS)

Alternative Name: Cholesterol Oxidase (choS Products)

Synonyms: SCD63.13 antibody, cholesterol oxidase antibody, GMC family oxidoreductase antibody, Cholesterol oxidase antibody, choS antibody, SCO4781 antibody, BTH_I0619 antibody, SARE_RS13770 antibody, Lcho_3191 antibody, Npun_R6526 antibody, Amir_2425 antibody, Caci_5961 antibody, Sros_4246 antibody, Kfla_6484 antibody, Snas_3535 antibody, Weevi_2091 antibody

Background:

Synonyms: choA antibody, choD antibody, choM antibody, choS antibody, COD antibody

Background: Cholesterol Oxidase is a bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. This enzyme participates in bile acid biosynthesis.

Gene Name: choA

Gene ID: 1215660

UniProt: P12676