Ascorbate Oxidase antibody (HRP)
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- Target See all Ascorbate Oxidase (AO) products
- Ascorbate Oxidase (AO)
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Reactivity
- Pumpkin
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Ascorbate Oxidase antibody is conjugated to HRP
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Application
- ELISA, Western Blotting (WB), Immunoprecipitation (IP)
- Cross-Reactivity (Details)
- Cross reactivity against Ascorbate Oxidase from other sources is unknown.
- Purification
- Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum and purified and partially purified Ascorbate Oxidase [cucurbita].
- Immunogen
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Immunogen: Ascorbate Oxidase [cucurbita]
Immunogen Type: Native Protein
- Isotype
- IgG
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- Application Notes
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Application Note: Anti-Ascorbate Oxidase has been assayed against 1.0 μg of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:11,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:3,000 - 1:10,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Ascorbate Oxidase (AO)
- Alternative Name
- Ascorbate Oxidase (AO Products)
- Synonyms
- AO antibody, ascorbate oxidase antibody, L-ascorbate oxidase antibody, AO antibody, LOC9310099 antibody
- Background
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Synonyms: L-ascorbate oxidase Ascorbase ASO EC=1.10.3.3
Background: Ascorbate Oxidase belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. The systematic name of this enzyme class is L-ascorbate:oxygen oxidoreductase.
Gene Name: AAO
- UniProt
- P24792
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