Stim2 antibody (C-Term)

Catalog No. ABIN5596756

Product Details anti-Stim2 Antibody

Target: Stim2 (Stromal Interaction Molecule 2 (Stim2))

Binding Specificity: C-Term

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Stim2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: STIM2 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100 % sequence homology.

Characteristics: Synonyms: sheep anti-Stim2 Antibody, STIM 2, STIM-2, Stromal interaction molecule 2

Purification: Anti-Stim II was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Stim II affinity purified antibody was prepared from whole sheep serum produced by repeated immunizations with a synthetic peptide near the C-terminus of human Stim II.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: Anti-Stim II antibody is tested for ELISA and immunohistochemistry and suitable for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~84 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Stim2

Target: Stim2 (Stromal Interaction Molecule 2 (Stim2))

Alternative Name: STIM2 (Stim2 Products)

Background: Background: Stim II antibody functions as a calcium sensor in the endoplasmic reticulum via its EF-hand domain. After depletion of Ca+2, it is thought to translocate from the endoplasmic reticulum to the plasma membrane to activate the calcium release-activated channel subunit. It may inhibit STIM1-mediated calcium influx. Anti-Stim II antibody is ideal for investigators interested in Signal Transduction research.

UniProt: Q9P246

Pathways: TCR Signaling, BCR Signaling