HMW HCP antibody

Catalog No. ABIN5596741

Product Details anti-HMW HCP Antibody

Target: HMW HCP (HMW Host Cell Proteins (HMW HCP))

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMW HCP antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli.

Cross-Reactivity (Details): Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.

Characteristics:

Synonyms: E.Coli Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, high molecular weight E.Coli HCP proteins antibody, HMW HCP

Background: Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli. This antibody has been anlyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.

Purification: This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli (BL21).

Immunogen Type: Native Protein

Application Details

Application Notes:

Western Blot Dilution: 1:500 - 1:1000

ELISA Dilution: 1:1000-1:2000

Comment:

Anti-HMW Host Cell Proteins Antibody detects over 250 E.coli HMW proteins and is suitable for western blotting and ELISA.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 77.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store HMW Host Cell Proteins Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HMW HCP

Target: HMW HCP (HMW Host Cell Proteins (HMW HCP))

Alternative Name: HMW E.Coli Host Cell Proteins