Hexokinase antibody (HRP)

Catalog No. ABIN5596731

Product Details anti-Hexokinase Antibody (HRP)

Target: Hexokinase

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Hexokinase from other tissues and species is expected based on sequence homology.

Purification: Anti-HEXOKINASE (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].

Immunogen:

Immunogen: Hexokinase [Yeast]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-HEXOKINASE (RABBIT) Antibody can be used in applications such as Immunohistochemistry (IHC), Western blot (WB) Immunofluorescence (IF) ELISA. Specific conditions should be optimized by researcher.

Western Blot Dilution: 1:500 - 1:1,000

ELISA Dilution: 1:1,000 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Hexokinase

Target: Hexokinase

Abstract: Hexokinase Products

Synonyms: CG32849 antibody, CG5443 antibody, Dmel\\CG32849 antibody, HK antibody, Hex antibody, anon-WO0140519.203 antibody, CG8094 antibody, CT22069 antibody, DM2 antibody, Dmel\\CG8094 antibody, Fk antibody, HEX antibody, HEX-3 antibody, HEX-C antibody, Hex-3 antibody, Hex-c antibody, HexC antibody, Hk antibody, CG3001 antibody, DM1 antibody, Dmel\\CG3001 antibody, HEX-A antibody, Hex-A:B antibody, HexA antibody, CG33102 antibody, CG54430 antibody, DHK-453 antibody, Dmel\\CG33102 antibody, HEX-t1 antibody, Dyak\\GE17799 antibody, GE17799 antibody, Hex-A antibody, dyak_GLEANR_19098 antibody, Dyak\\GE12255 antibody, GE12255 antibody, Hex-C antibody, dyak_GLEANR_12524 antibody, dsim_GLEANR_11078 antibody, DsimGD11104 antibody, GD11104 antibody, CG32849 gene product from transcript CG32849-RA antibody, Hexokinase C antibody, Hexokinase A antibody, CG33102 gene product from transcript CG33102-RA antibody, Phosphotransferase antibody, GE17799 gene product from transcript GE17799-RA antibody, GE12255 gene product from transcript GE12255-RA antibody, GD11104 gene product from transcript GD11104-RA antibody, hexokinase antibody, Hex-t2 antibody, Hex-C antibody, Hex-A antibody, Hex-t1 antibody, hxk-1 antibody, Dyak\Hex-A antibody, Dyak\Hex-C antibody, Dsim\Hex-C antibody, Smp_043030 antibody

Background:

Synonyms: DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody, HEX1, HKB

Background: HEXOKINASE Antibody is specific for hexokinase. Hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose. Anti Hexokinase is ideal for investigators involved in glucose energy metabolism, enzymes and AKT signaling research.

Gene Name: HXK2

Gene ID: 852639

UniProt: P04807