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Hexokinase 2 antibody (HRP)

Rockland 200-4359-0100 HK2 Reactivity: Saccharomyces cerevisiae WB, ELISA Host: Rabbit Polyclonal HRP
Rockland
Catalog No. ABIN5596731
Supplier Product No.: 200-4359-0100
  • Target See all Hexokinase 2 (HK2) Antibodies
    Hexokinase 2 (HK2)
    Reactivity
    • 73
    • 29
    • 24
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Saccharomyces cerevisiae
    Host
    • 64
    • 20
    Rabbit
    Clonality
    • 64
    • 20
    Polyclonal
    Conjugate
    • 53
    • 8
    • 7
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Hexokinase 2 antibody is conjugated to HRP
    Application
    • 68
    • 34
    • 33
    • 12
    • 11
    • 11
    • 9
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    200-4359-0100
    Supplier
    Rockland
    Purpose
    Hexokinase Antibody Peroxidase Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].
    Characteristics
    Synonyms: rabbit anti-Hexokinase antibody HRP conjugation, peroxidase conjugation rabbit anti-Hexokinase antibody, DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody, HEX1, HKB
    Purification
    Anti-HEXOKINASE (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Hexokinase [Yeast]

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product HK2 Primary Antibody
  • Application Notes

    Application Note: Anti-HEXOKINASE Peroxidase Conjugated Antibody has been tested by ELISA and western blot and can be used in applications such as Immunohistochemistry (IHC), and Immunofluorescence (IF). Specific conditions should be optimized by researcher.

    Western Blot Dilution: 1:500 - 1:1,000

    ELISA Dilution: 1:1,000 - 1:2,500

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer
    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    Hexokinase 2 (HK2)
    Alternative Name
    HXK2 (HK2 Products)
    Background
    Background: HEXOKINASE Antibody is specific for hexokinase. Hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose. Anti Hexokinase is ideal for investigators involved in glucose energy metabolism, enzymes and AKT signaling research.
    Gene ID
    852639
    UniProt
    P04806
    Pathways
    PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect
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