Glycerol Kinase antibody (HRP)

Catalog No. ABIN5596716

Product Details anti-Glycerol Kinase Antibody (HRP)

Target: Glycerol Kinase (GK)

Reactivity: Cellulomonas

Host: Goat

Clonality: Polyclonal

Conjugate: This Glycerol Kinase antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB)

Cross-Reactivity (Details): Cross reactivity against Glycerol Kinase from other sources is unknown.

Purification: Glycerol Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glycerol Kinase [Cellulomonas species].

Immunogen:

Immunogen: Glycerol Kinase [Cellulomonas species]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Glycerol Kinase has been assayed against 1.0 μg of Glycerol Kinase [Cellulomonas species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Glycerol Kinase

Target: Glycerol Kinase (GK)

Alternative Name: Glycerol Kinase (GK Products)

Synonyms: GK1 antibody, GKD antibody, D930012N15Rik antibody, GK antibody, CG18374 antibody, Dmel\\CG18374 antibody, dGyk antibody, ASTP antibody, Gyk antibody, gk1 antibody, gk2 antibody, gkd antibody, gyk antibody, Gk antibody, BmGK antibody, DKFZp469P1225 antibody, glycerol kinase antibody, Glycerol kinase 1 antibody, glycerol kinase S homeolog antibody, putative glycerol kinase 3 antibody, ATP:glycerol 3-phosphotransferase; glycerokinase; GK antibody, Glycerol kinase antibody, Fanconi anemia core complex associated protein 100 antibody, GK antibody, Gk antibody, Gk1 antibody, gk.S antibody, LOC705001 antibody, glpK antibody, Spico_1557 antibody, Trebr_0397 antibody, Halhy_5315 antibody, gk antibody, FAAP100 antibody

Background:

Synonyms: ATP glycerol 3 phosphotransferase antibody, GK antibody, GK antibody, GK1 antibody, GKD antibody, Glycerokinase antibody, Glycerol kinase antibody, Glycerol kinase deficiency antibody, HRP

Background: Glycerol Kinase is a key enzyme in the regulation of glycerol uptake and metabolism. Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis.

Gene Name: glpK

Gene ID: 6960048

UniProt: D0VZG4