Ferritin antibody (HRP)

Catalog No. ABIN5596710

Product Details anti-Ferritin Antibody (HRP)

Target: Ferritin (FE)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ferritin antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity (Details): Cross reactivity against Ferritin from other sources is unknown.

Purification: Anti-Ferritin (Human Spleen) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].

Immunogen:

Immunogen: Ferritin [Human Spleen]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Ferritin (Human Spleen) Antibody has been assayed against 1.0 μg of Ferritin [Human Spleen] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for Anti-Ferritin (Human Spleen) Antibody.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

IF Microscopy Dilution: User Optimized

Comment:

User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Ferritin

Target: Ferritin (FE)

Alternative Name: Ferritin (FE Products)

Synonyms: 1HCH antibody, BEST:LD36673 antibody, CG2216 antibody, Dmel\\CG2216 antibody, FER1 antibody, FER1HCH antibody, Fer-L antibody, Fer1 antibody, Fer1HC antibody, FerHCH antibody, HCH antibody, T21 antibody, T9 antibody, fer1 antibody, fer1hch antibody, l(3)00451 antibody, l(3)j10B4 antibody, SFerH-1 antibody, SOF-35 antibody, SOF-5L antibody, NV13011 antibody, ACP1 antibody, F8A5.24 antibody, F8A5_24 antibody, Ferritin 1 heavy chain homologue antibody, ferritin light chain antibody, ferritin antibody, chromosome 26 open reading frame, human C10orf131 antibody, hyaluronan and proteoglycan link protein 2 antibody, NAD(P)-linked oxidoreductase superfamily protein antibody, Fer1HCH antibody, H-1 antibody, YPDSF_1340 antibody, ftnA antibody, Ft antibody, C26H10orf131 antibody, HAPLN2 antibody, AT1G60730 antibody

Background:

Synonyms: Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH

Background: Ferritin is a intracellular protein that directly stores iron and regulates its release, specifically acting as a buffer for iron in humans. Ferritin is composed of 24 subunits and comes in two types, H and L. The H subunit detoxifies iron while the L subunit deals with long term storage of iron. Depending on the concentration of ferritin in the blood, different disorders and diseases could be present in an individual. Anti-Ferritin (Human Spleen) Antibody is ideal for investigators in Cancer, Immunology, and Cardiovascular Research.

Gene ID: 2495

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis