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Dextranase antibody (HRP)

Rockland 200-403-089-0100 Reactivity: Penicillium sp. WB, ELISA, IP Host: Rabbit Polyclonal HRP
Rockland
Catalog No. ABIN5596708
Supplier Product No.: 200-403-089-0100
  • Target See all Dextranase products
    Dextranase
    Reactivity
    Penicillium sp.
    Host
    • 10
    Rabbit
    Clonality
    • 10
    Polyclonal
    Conjugate
    • 5
    • 4
    • 1
    This Dextranase antibody is conjugated to HRP
    Application
    • 10
    • 8
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Supplier Product No.
    200-403-089-0100
    Supplier
    Rockland
    Purpose
    Dextranase Antibody Peroxidase Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species].
    Characteristics
    Synonyms: rabbit anti-Dextranase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Dextranase Antibody, dextranase, Alpha-1,6-glucan-6-glucanohydrolase, DEX
    Purification
    Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Dextranase [Penicillium species]

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Application Note: Anti-Dextranase Peroxidase has been tested by ELISA and western blot and assayed against 1.0 μg of Dextranase [Penicillium species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: User Optimized

    ELISA Dilution: 1:2,000 - 1:10,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    Dextranase
    Abstract
    Dextranase Products
    Background
    Background: Dextranase belongs to the glycosyl hydrolase 49 family. It's catalytic reaction is endohydrolysis of (1->6)-alpha-D-glucosidic linkages in dextran.
    UniProt
    P48845
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